Remove the lid from the first agar plate and place the exposed agar portion in or out of the building for the duration of todays lab. Aseptic techniques, antimicrobial substances and microbial growth as and a level duration. Sterile products and aseptic techniques for the pharmacy. Aseptic technique in order to study and characterize a microorganism, it is imperative to isolate it from all other organisms and maintain it in pure culture. Which action is an aseptic technique that would reduce the. Biology is a hands on science, and biology students are usually required to spend some time in the laboratory. The elements of aseptic technique are a sterile work area, good personal hygiene, sterile reagents and media, and sterile. You have free access to a large collection of materials used in a collegelevel introductory microbiology course. The word aseptic means to be free of harmful microorganisms. During the gowning and gloving of other team members, interaction among the team often sets the tone for the entire surgical procedure. Aseptic technique and the transfer of microorganisms, virtual amrita laboratories microbiology lab flashcards from quizlet when clinical samples are plated, a sterile swab is used to obtain the biological sample from the patient such as throat swab and to transfer that sample to a plate. Mar 05, 2020 biology is a hands on science, and biology students are usually required to spend some time in the laboratory. Aseptic technique is a basic laboratory technique that must be employed especially during microbiology laboratory session so as to prevent any contamination and affecting the accuracy of the result.
In the laboratory aseptic techniques are important so that you do not contaminate your culture, yourself or anyone around you. Here are some examples of how this technique is practiced. Many of the organisms can be opportunistic in their abilities to cause infection. Risk assessment consider the risk to either the patient or yourself of acquiring an infection as a result of the procedure. Streak the loop across the surface of the agar medium using the either the pattern shown in fig. Aseptic technique post lab quiz module 3 aseptic post lab q. However the solution requires water to be effective at doing its job. Media and equipment selection sterile work area for labs with cell culture hoods. Gowns are considered sterile only from waist to shoulders level in front and the sleeves. How to streak for isolated colonies sfic mixed cultures more than one species can be isolated using the streak plate technique. It is a method used to prevent contamination of wounds and. Why is 70% ethanol preferred for aseptic techniques. Aseptic technique requires at least a five minute hand washing. Durham tubes are used to detect the production of gases, such as co2 or n2, by microorganisms.
An opengloving technique would be used when performing urinary catheterization. The use of critical micro aseptic fields and a nontouch technique is essential to protect key parts and key sites. Learn microbiology lab aseptic technique with free interactive flashcards. The goal is to reach asepsis, which means an environment that is free of harmful microorganisms. The founder of the technique is considered to be joseph lister. Biology laboratory techniques wikibooks, open books for an. Aseptic technique is the effort taken to keep patients as free from hospital microorganisms as possible crow 1989. As a subject matter expert on good manufacturing practices for component preparation, maintaining sterility from the autoclave to the production area, and facilitating aseptic technique, he has been an instructor with the aseptic training institute ati since 2008, conducting trainings at their clayton, nc and university of cardiff, wales.
There are some general rules to follow for any aseptic technique. These streaking patterns allow you to obtain single isolated bacterial colonies originating from a single bacterium or arrangement of bacteria in order to avoid digging into the agar as you streak. View lab report aseptic technique lab answer sheet. Prevent contamination of the specific microorganism we are working with. A medical asepsis requires hands to be held downwards while rinsing, while surgical asepsis requires that. Cell culture protocol for proper aseptic technique to maintain a sterile environment and to prevent cell culture contamination. Lift the edge of the lid just enough to insert the loop 4. Plant geneticist, for example, sometimes spend time out in fields gathering plants, while molecular biologists may use complex equipment such. What is the role of aseptic techniques in a microbiology lab. Explain how to sterilize it, and how to distribute it in different formats. The tube is initially filled with the medium and then collects gas as the bacteria grow, creating a.
Rp2 microbiology aseptic technique practical and worksheets. View test prep aseptic technique post lab quiz from biology 1406 at odessa college. You dont want to go lower, because it will not kill the microorganisms you are trying to kill. Biology aseptic techniques a level core practical youtube. State the color and watersolubility of pigment seen on a plate culture of a chromogenic bacterium. Essential methods for maintaining, preparing and using cultures. If you contaminate yourselfwho knows what might happen. The 30% water is necessary to provide sufficient polarity to the solution. It includes both a risk assessment and the use of specific infection control measures. Jan 16, 2012 aseptic techniques, antimicrobial substances and microbial growth as and a level duration. Correct use and care of the laboratory equipment is considered a fundamental part of good laboratory technique. Part 1 of the book includes information on good microbiological laboratory practice, equipment, preparation of culture media, sterilisation and disinfection, inoculation, aseptic technique, incubation, cultures, contamination and working with moulds, bacteria and yeasts. Even if there is no visible sign of a tear, if a glove is thought to have been punctured, it should be changed.
May 11, 2012 use aseptic technique to inoculate sterile nutrient broth then incubate according to specifications of the bacterial strain. What are the principles of aseptic technique answers. Practical questions gcse biology single science revision bbc. Student practical instructions results table and questions exam questions exam answers card sorting activity other ideasmethods for the practical this is matched to the aqa required practical. Aseptic technique process aseptic technique is a framework for aseptic practice. Biology practicals and revision biology tutor 21,680 views. The aseptic techniques described here control the opportunities for contamination of cultures by microorganisms from the environment, or contamination of the environment by the microorganisms being handled. Choose from 500 different sets of microbiology lab aseptic technique flashcards on quizlet.
Aseptic technique and the transfer of microorganisms theory. Plant geneticist, for example, sometimes spend time out in fields gathering plants, while molecular biologists may use complex equipment such as dna sequencing. While this training provides extended career opportunities, it is a challenging and precise skill to learn. Make sure that you cite all external data sources, including your textbook. Aseptic technique microbiology resource center truckee. Apr 21, 2017 aseptic techniques aseptic techniques are the precautionary measures taken to prevent contamination of pure cultures and sterile laboratory equipment.
Contents about this resource inside front cover part 1. Updated in a brand new edition, this book covers the latest principles of aseptic technique, terms, methods, products and includes a howto on standard sterile product. Why are aseptic techniques important in the laboratory. Leftover cultures should be discarded at the end of the day. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Describe the different formats of media, plate, tube etc. Aug 23, 20 aseptic techniques, antimicrobial substances and microbial growth as and a level duration. An example of using aseptic techniques is when growing bacteria. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Cecile amadou bio 204 aseptic technique answer sheet prelaboratory questions 1.
Microorganisms in the lab atmosphere may come to rest on the desktop between classes. Produce nutrient agar na plates, na slants, and nutrient broths nbwhich will be used in. The next step is to inoculate the sterile agar with the microorganisms. Aseptic technique is intended to minimize contamination from all microorganisms. This manual, from the microbiology society, provides a basic introduction to microbiology, aseptic technique and safety. Aseptic technique is a method that prevents the introduction of unwanted organisms into an environment. If you contaminate your culture, your results will be inaccurate and wont be pure for that matter. Standard aseptic technique requires a main general aseptic field and may require nonsterile gloves only this can be dependent on clinician competence in performing the procedure.
Biology only q5 aseptic technique is used to prepare an uncontaminated culture containing only e. Aqa required practical activities practice questions. This resource is based around a practical task for aseptic technique. Practical activities are not just motivational and fun. Instructions for good laboratory practice and care of laboratory equipment. Surgical asepsis is protection against infection before, during, and after surgery by using sterile technique. Proper aseptic technique prevents contamination of cultures from. The type of activities a student will perform vary depending on the exact field they are in. Aseptic technique post lab quiz module 3 aseptic post. Microorganisms in the lab atmosphere may come to rest on the desktop between classes continue reading aseptic.
The simplest and most economical way to reduce contamination from airborne particles and aerosols e. In the microbiology laboratory, pure cultures of microorganisms are constantly being manipulated for the purpose of performing tests or simply for maintaining a viable culture. Sep 12, 2016 this resource is based around a practical task for aseptic technique. E book or pdf edited book email encyclopedia article govt. Prevent contamination of the room and personnel with the. Aseptic techniques and bacteria biology bibliographies in harvard style. Part of the pharmacy technician series, sterile products is a comprehensive book covering the complex practice of sterile product preparation and correct aseptic technique.
Aseptic technique is used for many procedures such as transferring cultures, inoculating media, and for performing a. Tmccs online microbiology resource center is designed to provide you with the resources to successfully complete the laboratory section of biology 251. Dilution streaking is when a bacterial stock is streaked with an inoculating loop onto solid media in a series of steps. After a biohazard spill is covered with paper towels and disinfectant solution, it must. Which action is an aseptic technique that would reduce the chance of bacteria on a lab table contaminating a lab notebook. Aseptic techniques aseptic techniques are the precautionary measures taken to prevent contamination of pure cultures and sterile laboratory equipment. Aseptic technique and the transfer of microorganisms.
You are about to begin topic 1, of cell culture techniques. Sterile product preparation and aseptic technique are advanced skills for pharmacy technicians. The purpose of aseptic technique is to reduce the number of harmful microorganisms. Aseptic an environment or procedure that is free of contamination by pathogens bauman, robert w. A sterile field is a microorganism free area that can receive sterile supplies. Biology laboratory techniques wikibooks, open books for.
Find aseptic technique lesson plans and teaching resources. Aseptic sterile technique used in microbiology laboratory. Aseptic technique refers to how doctors and nurses prevent the spread of infection. Laboratory exercises in microbiology mclaughlin and petersen iv.
Aseptic technique is essentially the backbone of microbiology. Aseptic technique, designed to provide a barrier between the microrganisms in the environment and the sterile cell culture, depends upon a set of procedures to reduce the probability of contamination from these sources. Use aseptic technique to inoculate sterile nutrient broth then incubate according to specifications of the bacterial strain. It is a fundamental skill for working in a microbiology laboratory. Media, incubation, and aseptic technique objectives 1. In the microbiology lab we use aseptic technique to. An introduction to microbiology, aseptic technique and safety preparation safety guidelines 1. Aseptic technique and transfer of microorganisms biology.
This book, however, has been designed to guide the student through with ease, as each theory builds on those presented in earlier chapters. Since microorganism can replicated rapidly, disposal of contaminants must be done properly so as to protect both the equipments and the health of. In order to study and characterize a microorganism, it is imperative to isolate it from all other organisms and maintain it in pure culture. Biology essay writing service male student reading book. Only sterile items are used within the sterile field.
Working within the barrier products solutions group at steris, he has over 15 years experience in the industry, identifying and providing solutions for pharmaceutical and biotechnology clients around the globe with needs related to process improvements in component preparation, maintaining sterility, particulate reduction and aseptic technique. Aseptic techniques are the precautionary measures taken to prevent contamination of pure cultures and sterile laboratory equipment. In order to illustrate that microorganisms are all around us and to demonstrate the necessity for proper aseptic technique, contaminate three trypticase soy agar plates as follows. Biology practicals and revision biology tutor 21,928 views. Jun 12, 2017 aseptic technique is essentially the backbone of microbiology. The basics an introduction to microbiology, aseptic technique and safety preparation safety guidelines 1 risk assessment 2.
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